;jselhsqmbc ;written by Teo parella & Josep Saurí - 151112 ;2D J-selHSQMBC-IPAP experiment ;Simultaneous Measurement of two different J(CHA) and J(CHB) as ;well as J(HAHB) using IPAP and E.COSY principles #include #include #include "p2=p1*2" "d4=1s/(cnst2*4)" "d22=p3" "d0=3u" "DELTA2=d4-larger(p12,p14)/2-50u-p16-d16" "DELTA3=d4-larger(p12,p14)/2-50u-p16-d16-50u-p16-d16" "DELTA=p16+d16+50u+p2+d0*2+50u" "in20=in0*cnst16" "d20=3u" 1 ze 2 d1 pl1:f1 pl2:f2 (p3 ph3):f2 50u UNBLKGRAD p16:gp6 d16 3 (p1 ph1) 50u p16:gp3 d16 DELTA2 pl0:f2 pl0:f1 4u (center (p12:sp2 ph1) (p14:sp3 ph6):f2 ) 4u DELTA2 pl2:f2 pl1:f1 50u p16:gp3 d16 p28 ph1 (p1 ph2) (p3 ph3):f2 ;starts selective j-resolved d20 (center (p13:sp4 ph1) (p24:sp7 ph6):f2 ) d20 ;ends d0 (p2 ph5) d0 50u p16:gp1*EA d16 pl0:f2 d12 (p24:sp7 ph14):f2 DELTA pl2:f2 (p3 ph4):f2 50u p16:gp4 d16 pl0:f1 if "cnst25==0" { (p11:sp1 ph2):f1 } else { (p11:sp1 ph1):f1 } 50u p16:gp5 d16 DELTA2 pl0:f2 if "cnst25==0" { (center (p12:sp2 ph1) (p14:sp3 ph1):f2 ) } else { (p12:sp2 ph1) } 4u DELTA3 pl2:f2 pl1:f1 50u p16:gp5 d16 50u p16:gp2 d16 BLKGRAD if "cnst25==0" { (p3 ph1):f2 } else { d22 } go=2 ph31 d1 mc #0 to 2 F1EA(igrad EA, id0 & id20 & ip3*2 & ip6*2 & ip31*2) exit ph1=0 ph2=1 ph3=0 2 ph4=0 0 2 2 ph5=0 ph6=0 ph14=0 0 2 2 ph31=0 2 2 0 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;sp1: f1 channel - shaped pulse 90 degree for selective excitation A ;sp2: f1 channel - shaped pulse 180 degree for selective inversion A ;sp3: f2 channel - shaped pulse 180 degree for broadband inversion ;sp4: f1 channel - shaped pulse 180 degree for selective inversion B ;sp7: f2 channel - shaped pulse 180 degree for refocussing ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p11: f1 channel - 90 degree shaped pulse for selective excitation A ;p12: f1 channel - 180 degree shaped pulse for selective inversion A ;p13: f1 channel - 180 degree shaped pulse for selective inversion B ;p14: f2 channel - 180 degree shaped pulse for inversion ;p16: homospoil/gradient pulse ;p22: f3 channel - 180 degree high power pulse ;p24: f2 channel - 180 degree shaped pulse for broadband refocussing ;p28: f1 channel - trim pulse ;d0 : incremented delay (2D) [3 usec] ;d20 : incremented delay (2D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d4 : 1/(4J)XH ;d16: delay for homospoil/gradient recovery ;cnst2: = nJ(XH) ;cnst16: scaling k factor ;cnst25: 0 for IP and 1 for AP ;inf1: 1/SW(X) = 2 * DW(X) ;in0: 1/(2 * SW(X)) = DW(X) ;in20: = in0 ;nd0: 2 ;NS: 2 * n ;DS: >= 8 ;td1: number of experiments ;FnMODE: echo-antiecho ;for z-only gradients: ;gpz1: 80% ;gpz2: 20.1% ;gpz3: 33% ;gpz4: 50% ;gpz5: 11% ;gpz6: 17% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100 ;gpnam4: SINE.100 ;gpnam5: SINE.100 ;gpnam6: SINE.100