;Pulse Program Code for Bruker: ;pp:hobs_tocsy ;2D Experiment ;HOmodecoupled Band-Selective TOCSY experiment (HOBS-TOCSY) ; based on the instant Zangger-Sterk experiment ;L. Castaņar, P. Nolis, A. Virgili & T. Parella, ; Chem. Eur. J (2013) DOI:chem.201303235 ;avance-version (07/0/2013) ;Topspin2.1 & Topspin3.1 #include #include #include #include dwellmode explicit "d12=20u" "d20=2m" "d21=3m" "d2=aq/l0" "d3=d2/2" "l2=l0-1" "p2=2*p1" "in0=inf1" "d0=in0/2-p1*4/3.1416" "FACTOR1=(d9/(p6*115.112))/2" "l1=FACTOR1*2" 1 ze 2 2m d1 50u UNBLKGRAD 3 d12 pl1:f1 p1 ph1 d0 p1 ph2 d20 pl10:f1 ;begin DIPSI2 4 p6*3.556 ph23 p6*4.556 ph25 p6*3.222 ph23 p6*3.167 ph25 p6*0.333 ph23 p6*2.722 ph25 p6*4.167 ph23 p6*2.944 ph25 p6*4.111 ph23 p6*3.556 ph25 p6*4.556 ph23 p6*3.222 ph25 p6*3.167 ph23 p6*0.333 ph25 p6*2.722 ph23 p6*4.167 ph25 p6*2.944 ph23 p6*4.111 ph25 p6*3.556 ph25 p6*4.556 ph23 p6*3.222 ph25 p6*3.167 ph23 p6*0.333 ph25 p6*2.722 ph23 p6*4.167 ph25 p6*2.944 ph23 p6*4.111 ph25 p6*3.556 ph23 p6*4.556 ph25 p6*3.222 ph23 p6*3.167 ph25 p6*0.333 ph23 p6*2.722 ph25 p6*4.167 ph23 p6*2.944 ph25 p6*4.111 ph23 lo to 4 times l1 ;end DIPSI2 d12 pl1:f1 p1 ph3 p16:gp1 d16 d12 pl0:f1 (p12:sp2 ph3) p16:gp1 d16 d12 pl1:f1 ACQ_START(ph30,ph31) 0.05u DWL_CLK_ON 0.1u REC_UNBLK d3:r 0.05u DWL_CLK_OFF 0.1u REC_BLK p16:gp2 d16 pl1:f1 p2 ph6 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph7) p16:gp3 d16 5 0.05u DWL_CLK_ON 0.1u REC_UNBLK d2:r 0.05u DWL_CLK_OFF 0.1u REC_BLK p16:gp2 d16 pl1:f1 p2 ph6 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph7) p16:gp3 d16 lo to 5 times l2 0.05u DWL_CLK_ON 0.1u REC_UNBLK d3 30m 0.05u DWL_CLK_OFF 0.1u REC_BLK rcyc=2 2m mc #0 to 2 F1PH(ip1, id0) exit ph0=0 ph1=0 2 ph2=0 0 0 0 2 2 2 2 ph3=0 0 2 2 ph23=3 ph25=1 ph6=0 2 ph7=0 2 ph30=0 ph31=0 2 2 0 2 0 0 2 ;pl1 : f1 channel - power level for pulse (default) ;pl10: f1 channel - power level for TOCSY-spinlock ;p1 : f1 channel - 90 degree high power pulse ;p6 : f1 channel - 90 degree low power pulse ;p12 : f1 channel - 180 degree band-selective pulse [ms] ;p16: homospoil/gradient pulse [1 ms] ;d0 : incremented delay (2D) ;d1 : relaxation delay; 1-5 * T1 ;d9 : TOCSY mixing time ;d12: delay for power switching [20 usec] ;d16: delay for homospoil/gradient recovery ;d20: first z-filter delay [2 msec] ;d21: second z-filter delay [3 msec] ;l1: loop for DIPSI cycle: ((p6*115.112) * l1) = mixing time ;l2: number of concatenated loops ;inf1: 1/SW = 2 * DW ;in0: 1/(1 * SW) = 2 * DW ;nd0: 1 ;NS: 8 * n ;DS: 16 ;td1: number of experiments ;FnMODE: States-TPPI, TPPI, States or QSEQ ;spoffs0: presat offset ;sp0: presat power (55dB) ;sp2: f1 channel - shaped pulse power level for band-selective excitation ;spnam2: shaped pulse for selective excitation [REBURP]