;Pulse Program Code for Bruker: ;pp:hobsproject ;1D Experiment ;HOmodecoupled Band-Selective Periodic Refocusing of J Evolution by Coherence Transfer NMR experiment (HOBS-PROJECT) ;Instant homonuclear decoupling is used during acquisition ;T2 measurement ;L. Castaņar, P. Nolis, A. Virgili & T. Parella, ; J. Magn. Reson.(2014)244,30-35 ; DOI:10.1016/j.jmr.2014.04.003 ;Avance III version (13/07/2013) ;Topspin3.1 #include #include #include #include "p2=p1*2" "d12=20u" "DELTA1=d20-p1*2/3.1416" "DELTA2=d20-p16-d16-de" "DELTA3=d20-p16-d16" "acqt0=0" dwellmode explicit "d2=aq/l0" "d3=d2/2" "l1=l0-1" 1 ze 2 30m d1 50u UNBLKGRAD d12 pl1:f1 p1 ph1 DELTA1 p2 ph2 d20 3 d20 p2 ph2 d20 p1 ph2 d20 p2 ph2 d20 lo to 3 times l4 DELTA3 pl0:f1 p16:gp1 d16 p12:sp2:f1 ph2 p16:gp1 d16 DELTA2 ;starts HOBS ACQ_START(ph30,ph31) 0.05u setrtp1|0 0.1u setrtp1|5 d3:r 0.1u setrtp1^5 0.05u setrtp1^0 p16:gp2 d16 pl1:f1 p2 ph4 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph3) p16:gp3 d16 4 0.05u setrtp1|0 0.1u setrtp1|5 d2:r 0.1u setrtp1^5 0.05u setrtp1^0 p16:gp2 d16 pl1:f1 p2 ph4 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph3) p16:gp3 d16 lo to 4 times l1 0.05u setrtp1|0 0.1u setrtp1|5 d3 5m 0.1u setrtp1^5 0.05u setrtp1^0 rcyc=2 wr #0 exit ph1=0 ph2=1 ph4=0 ph3=0 ph30=0 ph31=0 ;pl1 : f1 channel - power level for pulse (default) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p12 : f1 channel - 180 degree band-selective pulse [ms] ;sp2: f1 channel - shaped pulse power level for band-selective excitation ;spnam2: shaped pulse for selective excitation [REBURP] ;d1 : relaxation delay; 1-5 * T1 [10 sec] ;d12: delay for power switching [20 usec] ;p16: homospoil/gradient pulse [500 us] ;d20: fixed echo time to allow elimination of J-mod. effects ;l1: number of concatenated loops for homodecoupling ;l4: loop for T2 filter [4 - 20] ;NS: 1 * n, total number of scans: NS * TD0 ;DS: 0 ;for z-only gradients: ;gpz1: 76% ;gpz2: 41% ;gpz3: 63% ;use gradient files: ;gpnam1: SMSQ10.100 ;gpnam2: SMSQ10.100 ;gpnam3: SMSQ10.100