;Pulse Program Code for Bruker: ;pp:hobsir2d ;2D Experiment ;HOmodecoupled Band-Selective Inversion Recovery NMR experiment (HOBS-IR) ;Instant homonuclear decoupling is used during acquisition ;T1 measurement using inversion recovery ;L. Castaņar, P. Nolis, A. Virgili & T. Parella, ; J. Magn. Reson.(2014)244,30-35 ; DOI:10.1016/j.jmr.2014.04.003 ;Avance III version (17/07/2013) ;Topspin3.1 #include #include #include #include dwellmode explicit "p2=p1*2" "d2=aq/l0" "d3=d2/2" "d11=30m" "l1=l0-1" "acqt0=-p1*2/3.1416" 1 ze 2 d1 pl1:f1 50u UNBLKGRAD p2 ph1 vd pl1:f1 p1 ph2 d16 pl0:f1 p16:gp1 (p12:sp2 ph3) p16:gp1 d16 ;starts HOBS ACQ_START(ph30,ph31) 0.05u setrtp1|0 0.1u setrtp1|5 d3:r 0.1u setrtp1^5 0.05u setrtp1^0 p16:gp2 d16 pl1:f1 p2 ph4 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph3) p16:gp3 d16 3 0.05u setrtp1|0 0.1u setrtp1|5 d2:r 0.1u setrtp1^5 0.05u setrtp1^0 p16:gp2 d16 pl1:f1 p2 ph4 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph3) p16:gp3 d16 lo to 3 times l1 0.05u setrtp1|0 0.1u setrtp1|5 d3 5m 0.1u setrtp1^5 0.05u setrtp1^0 rcyc=2 d11 wr #0 if #0 ivd lo to 1 times td1 exit ph1=0 2 ph2=0 0 2 2 1 1 3 3 ph3=0 ph4=0 ph30=0 ph31=0 0 2 2 1 1 3 3 ;pl1 : f1 channel - power level for pulse (default) ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p12 : f1 channel - 180 degree band-selective pulse [ms] ;p16: homospoil/gradient pulse [500 us] ;sp2: f1 channel - shaped pulse power level for band-selective excitation ;spnam2: shaped pulse for selective excitation [REBURP] ;d1 : relaxation delay; 1-5 * T1 [10 sec] ;d11: delay for disk I/O [30 msec] ;d16: delay for homospoil/gradient recovery [20u] ;vd : variable delay, taken from vd-list ;NS: 1 * n ;DS: 0 ;td1: number of experiments = number of delays in vd-list ;FnMODE: undefined ;l1: number of concatenated loops ;define VDLIST ;this pulse program produces a ser-file (PARMOD = 2D) ;for z-only gradients: ;gpz1: 76% ;gpz2: 41% ;gpz3: 63% ;use gradient files: ;gpnam1: SMSQ10.100 ;gpnam2: SMSQ10.100 ;gpnam1: SMSQ10.100