Bruker pulse program: ;teo_hsqmbc-cosy #include #include #include "p2=p1*2" "p4=p3*2" "d0=3u" "d6=1s/(cnst13*4)" "DELTA=p2+d0*2+p16+d16" "DELTA1=d6-larger(p2,p14)/2" "DELTA2=p16+d16+4u" "DELTA3=d6-larger(p2,p14)/2-p16-d16" 1 ze 2 d1 do:f2 3 (p1 ph1) DELTA1 UNBLKGRAD d12 pl0:f2 (center (p2 ph1) (p14:sp3 ph6):f2 ) d12 DELTA1 pl2:f2 p28 ph1 (p1 ph2) (p3 ph3):f2 d0 (p2 ph5) d0 p16:gp1*EA d16 pl0:f2 (p24:sp7 ph4):f2 DELTA pl2:f2 (p3 ph4):f2 (p1 ph1) if "cnst25==0" { DELTA1 pl0:f2 (center (p2 ph1) (p14:sp3 ph1):f2 ) DELTA1 pl2:f2 } else { d6 (p2 ph1) d6 } (p1 ph2):f1 DELTA2 p2 ph1 p16:gp2 d16 pl12:f2 4u BLKGRAD go=2 ph31 cpd2:f2 d1 do:f2 mc #0 to 2 F1EA(igrad EA, id0 & ip3*2 & ip6*2 & ip31*2) exit ph1=0 ph2=1 ph3=0 2 ph4=0 ph5=0 ph6=0 ph7=2 ph23=3 ph25=1 ph31=0 2 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;sp3: f2 channel - shaped pulse 180 degree for inversion ;spnam3: Crp60,0.5,20.1 ;sp7: f2 channel - shaped pulse 180 degree for refocussing ;spnam7: Crp60comp.4 or bandselective pulse ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p4 : f2 channel - 180 degree high power pulse ;p14: f2 channel - 180 degree shaped pulse for inversion ; = 500usec for Crp60,0.5,20.1 ;p16: homospoil/gradient pulse ;p24: f2 channel - 180 degree shaped pulse for refocussing ; = 2msec for Crp60comp.4 ;d0 : incremented delay (2D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d6 : delay for evolution of long range couplings (1/2Jlr) ;d16: delay for homospoil/gradient recovery ;cnst13: = J(XH) long range ;cnst25: = 0 (IP) or 1 (AP) ;cnst26: = 1 (HSQMBC) or 0 (HSQMBC-TOCSY) ;in0: 1/(2 * SW(X)) = DW(X) ;nd0: 2 ;NS: 1 * n ;DS: >= 16 ;td1: number of experiments ;FnMODE: echo-antiecho ;cpd2: decoupling according to sequence defined by cpdprg2 ;pcpd2: f2 channel - 90 degree pulse for decoupling sequence ;use gradient ratio: gp 1 : gp 2 ; 80 : 20.1 for C-13 ; 80 : 8.1 for N-15 ;for z-only gradients: ;gpz0: 3 ;gpz1: 80% ;gpz2: 20.1% for C-13, 8.1% for N-15 ;gpz3: 30% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100 ;gpnam3: SINE.100