;Pulse Program Code for Bruker: ;pp:hobs ;1D Experiment ;HOmodecoupled Band-Selective NMR experiment (HOBS) ; based on the instant Zangger-Sterk experiment ;Method for fast acquisition and full sensitive homodecoupled band-selective spectra ;N.H. Meyer and K. Zangger. ; Angew. Chem. Int. Ed. (2013) 52, 7143-7146 ;L. Castaņar, P. Nolis, A. Virgili & T. Parella, ; Chem. Eur. J (2013)19,17283-17286 ; DOI:chem.201303235 ;Avance III version (01/07/2013) ;Topspin2.1 & Topspin3.1 #include #include #include #include dwellmode explicit "d12=6.5u" "d2=aq/l0" "d3=d2/2" "l1=l0-1" "p2=2*p1" 1 ze 2 3m d1 50u UNBLKGRAD d12 pl1:f1 p1 ph1 p16:gp1 d16 pl0:f1 (p12:sp2 ph1) p16:gp1 d16 ACQ_START(ph30,ph31) 0.05u setrtp1|0 0.1u setrtp1|5 d3:r 0.1u setrtp1^5 0.05u setrtp1^0 p16:gp2 d16 pl1:f1 p2 ph2 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph3) p16:gp3 d16 3 0.05u setrtp1|0 0.1u setrtp1|5 d2:r 0.1u setrtp1^5 0.05u setrtp1^0 p16:gp2 d16 pl1:f1 p2 ph2 p16:gp2 d16 p16:gp3 d16 pl0:f1 (p12:sp2 ph3) p16:gp3 d16 lo to 3 times l1 0.05u setrtp1|0 0.1u setrtp1|5 d3 30m 0.1u setrtp1^5 0.05u setrtp1^0 rcyc=2 wr #0 exit ph1=0 2 2 0 1 3 3 1 ph2=0 2 ph3=0 2 ph30=0 ph31=0 2 2 0 1 3 3 1 ;pl1 : f1 channel - power level for pulse (default) ;p1 : f1 channel - high power pulse ;p12 : f1 channel - 180 degree band-selective pulse [ms] ;p16: homospoil/gradient pulse [500 us] ;sp2: f1 channel - shaped pulse power level for band-selective excitation ;spnam2: shaped pulse for selective excitation [REBURP] ;d1 : relaxation delay [1s] (only one scan is recorded) ;d16: delay for homospoil/gradient recovery [20us] ;NS: 1 * n ;DS: 0 ;l1: number of concatenated loops ;for z-only gradients: ;gpz1: 17% ;gpz2: 41% ;gpz3: 63% ;use gradient files: ;gpnam1: SMSQ10.100 (Topspin3.1) ; SINE.100 (Topspin2.1) ;gpnam2: SMSQ10.100 (Topspin3.1) ; SINE.100 (Topspin2.1) ;gpnam3: SMSQ10.100 (Topspin3.1) ; SINE.100 (Topspin2.1)