;Pulse Program Code for Bruker:

;pp:perfect_hsqc
;2D Experiment
;F2-heterocoupled perfect CLIP-HSQC with perfect-echo INEPT periods to obtain pure in-phase cross peaks.
;2D H-1/X correlation via inept transfer
;phase sensitive using Echo/Antiecho-TPPI gradient selection



;L. Castañar, E. Sistaré, A. Virgili, R. T. Williamson, T. Parella
;   Magn. Reson. Chem.(2015) 52, 115-119
;   DOI 10.1002/mrc.4149


;Bruker AvanceIII 
;Topspin3.1


#include <Avance.incl>
#include <Grad.incl>
#include <Delay.incl>


"p2=p1*2"
"p4=p3*2"
"d0=3u"
"d4=1s/(cnst2*4)"
"d11=30ms"

"in0=inf1/2"

"DELTA=p2+d0*2+p16+d16"
"DELTA1=d4-larger(p2,p14)/2"
"DELTA2=d4-larger(p2,p14)/2-p16-d16-4u"


1 ze
  d11 pl12:f2
2 d1 do:f2
3 (p1 ph1)

  d4 UNBLKGRAD
  (p2 ph1)
  d4 
  (p1 ph2)
  DELTA1 pl0:f2
  (center (p2 ph1) (p14:sp3 ph6):f2 )
  DELTA1 pl2:f2
  (p1 ph2) 
  p16:gp3
  (p3 ph3):f2

  d0 
  (p2 ph5)
  d0
  p16:gp1*EA
  d16 pl0:f2
  (p24:sp7 ph4):f2
  DELTA pl2:f2
  
  (p3 ph4):f2
  (p1 ph1) 
  d4 
  (p2 ph1)
  d4 
  (p1 ph2)
  DELTA1 pl0:f2
  (center (p2 ph1) (p14:sp3 ph1):f2 )
  DELTA2 pl12:f2  
  p16:gp2
  d16 
  4u BLKGRAD
   
  go=2 ph31 cpd2:f2
  d1 do:f2 mc #0 to 2 
     F1EA(igrad EA, id0 & ip3*2 & ip6*2 & ip31*2)
exit
   

ph1=0 
ph2=1
ph3=0 2
ph4=0 
ph5=0 
ph6=0
ph31=0 2


;pl0 : 120dB
;pl1 : f1 channel - power level for pulse (default)
;pl2 : f2 channel - power level for pulse (default)
;sp3: f2 channel - shaped pulse 180 degree
;spnam3: shaped pulse for 180 degree for inversion [Crp60,0.5,20.1] 
;sp7: f2 channel - shaped pulse 180 degree for refocussing
;spnam7: shaped pulse for 180 degree for refocussing [Crp60comp.4]  
;p1 : f1 channel -  90 degree high power pulse
;p2 : f1 channel - 180 degree high power pulse
;p3 : f2 channel -  90 degree high power pulse
;p4 : f2 channel - 180 degree high power pulse
;p14: f2 channel - 180 degree shaped pulse for inversion   [500usec]
;p16: homospoil/gradient pulse [1 ms]
;p24: f2 channel - 180 degree shaped pulse for refocussing   [2msec]
;d0 : incremented delay (2D)                                 [3usec]
;d1 : relaxation delay; 1-5 * T1
;d4 : 1/4*J(XH)
;d16: delay for homospoil/gradient recovery
;cnst2: = J(XH) 
;in0: 1/(2 * SW(X)) = DW(X)
;nd0: 2
;NS: 2 * n
;DS: >= 2
;td1: number of experiments
;FnMODE: echo-antiecho


;use gradient ratio:	gp 1 : gp 2
;			  80 : 20.1    for C-13
;			  80 :  8.1    for N-15

;for z-only gradients:
;gpz1: 80%
;gpz2: 20.1% for C-13, 8.1% for N-15
;gpz3: 33%

;use gradient files:   
;gpnam1: SMSQ10.100
;gpnam2: SMSQ10.100
;gpnam3: SMSQ10.100


;$Id: lau_perfect_hsqcetgpsp,v 1.3 2014/03/27 10:38:471 $