Bruker pulse program: ;2jhh-res ;2JHH-resolved HSQC #include #include #include #include "d22=d2-larger(p2,p14)/2" "p2=p1*2" "d0=3u" "d20=3u" "d21=3u" "d2=1s/(cnst2*2)" "d4=1s/(cnst2*4)" "in0=inf1/2" "in20=in0*cnst20" "in21=in0*cnst21" "DELTA=p16+d16+p2+d21*2-4u" "DELTA2=d4-p14/2-4u" "DELTA3=p16+d16+50u" "DELTA4=d4-p14/2-4u-p16-d16" "DELTA5=d2-larger(p2,p14)/2" "count=(aq/d19)-1" "acqt0=0" baseopt_echo 1 ze 2 d1 do:f2 d12 pl1:f1 3 (p1 ph1) DELTA2 pl0:f2 4u (center (p2 ph1) (p14:sp3 ph6):f2 ) 4u p28 ph1 DELTA2 pl2:f2 UNBLKGRAD (center (p1 ph2) (p3 ph3):f2 ) d21 (p2 ph1) d21 p16:gp1*EA d16 pl0:f2 (p24:sp7 ph4):f2 4u DELTA pl2:f2 (center (p1 ph1) (p3 ph4):f2 ) DELTA2 pl0:f2 4u (center (p2 ph1) (p14:sp3 ph1):f2 ) 4u DELTA2 d20 p1 ph1 DELTA5 pl0:f2 (center (p2 ph1) (p14:sp3 ph6):f2 ) DELTA5 pl2:f2 p1 ph1 (p4 ph1):f2 ;OPTIONAL for specific 2JHH-J-resolved spectrum d20 p1 ph2 d12 pl5:f1 300u gron0 p32:sp29:f1 ph1 100u groff d16 d12 pl1:f1 p1 ph1 DELTA3 p2 ph1 50u p16:gp2 d16 BLKGRAD d12 pl12:f2 go=2 ph31 cpd2:f2 d1 do:f2 mc #0 to 2 F1EA(calgrad(EA), caldel(d0, +in0) & caldel(d20, +in20) & caldel(d21, +in21)) exit ph1=0 ph2=1 ph3=0 2 ph4=0 ph6=0 ph7=2 ph15=1 ph16=2 ph17=3 ph25=2 ph26=3 ph27=0 ph30=0 ph31=0 2 ;pl0 : 120dB ;pl1 : f1 channel - power level for pulse (default) ;pl2 : f2 channel - power level for pulse (default) ;pl10: f1 channel - power level for TOCSY-spinlock ;sp3: f2 channel - shaped pulse 180 degree ;spnam3: Crp60,0.5,20.1 ;sp7: f2 channel - shaped pulse (180degree refocussing) ;spnam7: Crp60comp.4 ;p1 : f1 channel - 90 degree high power pulse ;p2 : f1 channel - 180 degree high power pulse ;p3 : f2 channel - 90 degree high power pulse ;p14: f2 channel - 180 degree shaped pulse for inversion ; = 500usec for Crp60,0.5,20.1 ;p16: homospoil/gradient pulse [1 msec] ;p24: f2 channel - 180 degree shaped pulse for refocussing ; = 2msec for Crp60comp.4 ;d0 : incremented delay (2D) [3 usec] ;d1 : relaxation delay; 1-5 * T1 ;d2 : 1/(2J(XH)) ;d4 : 1/(4J(XH)) ;d16: delay for homospoil/gradient recovery ;cnst2: = J(XH) ;cnst15: = conventional(0)/pure shift(1) ;cnst14: = conventional(0)/2JHH-resolved(1) ;cnst17: = -0.5 for Crp60comp.4 ;cnst20: = J(scale) factor ;cnst21: = Correlation factor ;in0: 1/(2 * SW(X)) = DW(X) ;nd0: 2 ;NS: 2 * n ;DS: >= 16 ;td1: number of experiments ;FnMODE: echo-antiecho ;use gradient ratio: gp 1 : gp 2 : gp 0 ; 80 : 20.1 : 11 for C-13 ;for z-only gradients: ;gpz1: 80% ;gpz2: 20.1% for C-13 ;gpz0: 11% ;use gradient files: ;gpnam1: SINE.100 ;gpnam2: SINE.100