Broadband homodecoupled Time-Shared 1H-13C and 1H-15N HSQC experiments

Nolis, P., Motiram-Corral, K., Pérez-Trujillo, M., & Parella, T. (2018). Broadband homodecoupled Time-Shared 1H-13C and 1H-15N HSQC experiments. Journal Magnetic Resonance. DOI: 10.1016/j.jmr.2018.11.005

The concepts of pure-shift NMR and time-shared NMR are merged in a single NMR experiment. A 13C/15N time-shared version of the real-time BIRD-based broadband homodecoupled HSQC experiment is described. This time-efficient approach affords simultaneously 1H-13C and 1H-15N pure-shift HSQC spectra in a single acquisition, while achieving substantial gains in both sensitivity and spectral resolution. We also present a related 13C/15N-F2-coupled homodecoupled version of the CLIP-HSQC experiment for the simultaneous measurement of 1JCH and 1JNH from the simplified doublets observed along the direct dimension. Finally, a novel J-resolved HSQC experiment has been designed for the simple and automated determination of both 1JCH/1JNH from a 2D J-resolved spectrum.

 

© 2018, Motiram-Corral Kumar. All rights reserved by the author(s) and Universitat Autonoma de Barcelona except for texts and images already copyrighted by third parties (e.g. journal publishers) and used here under the fair use provision.