This hyphenated technique combines HPLC with three complementary detection techniques – NMR Spectroscopy, Mass Spectrometry and Diode Array Detection. It is a highly rich information source, which can be obtained by one-shot analysis.
There are three different modes of work: Flow-NMR, Stop-Flow and SPE-storage; and depending on the scientific problem one or another will be the most adequate. Continue reading HPLC-DAD-(SPE)-NMR/MS II: fundamentals and working modes
The combination of liquid chromatography with three complementary detection techniques – NMR Spectroscopy, Mass Spectrometry and Diode Array Detection, provides highly rich information in a one-shot analysis.
The possibility of a post-chromatography storage /concentration of the chromatographic peaks of interest, using a SPE (Solid Phase Extraction) system, together with the use of cool probes, increases the sensitivity of the NMR detection.
Continue reading HPLC-DAD-(SPE)-NMR/MS I: all in one technique for the analysis of mixtures
There is a great interest in understanding the biochemical basis for the reported beneficial health effects of particular diets or food groups. Characterising the wide variety of nutrients in the things we eat and drink in detail is a substantial analytical challenge, especially when numerous sets of molecules in such mixtures may have very similar chemical structures.
Continue reading Identification of compounds of biological significance in foodstuffs by LC-NMR/MS. Olive oil
“Separation and Identification of Phenolic Compounds of Extra-Virgin Olive Oil from Olea Europaea L. by HPLC-DAD-SPE-NMR/MS. Identification of a New Diastereoisomer of the Aldehydic Form of Oleuropein Aglycone”, by Míriam Pérez-Trujillo, Ana María Gómez-Caravaca, Antonio Segura-Carretero, Alberto Fernández-Gutiérrez, Teodor Parella; Journal of Agricultural and Food Chemistry 58, 9129-9136 (2010). DOI: 10.1021/jf101847e
The phenolic fraction of a monovarietal extra virgin olive oil (EVOO) from Olea europaea L. var. Cornezuelo was studied by the hyphenated HPLC-DAD-SPE-NMR/MS techniques. This survey led to the identification of 25 main compounds. One was identified as a new diastereoisomer of the aldehydic form of oleuropein aglycone (AOA) and characterized by 1D and 2D NMR techniques. Continue reading Analysis of phenolic compounds in Extra Virgin Olive Oil by LC-NMR/MS. Differentiation and identification of diastereoisomeric molecules