“P.E.HSQMBC: Simultaneous measurement of proton–proton and proton–carbon coupling constants” by Josep Saurí, Pau Nolis, Laura Castañar, Albert Virgili and Teodor Parella. Journal of Magnetic Resonance 224 (2012) 101–106. DOI: 10.1016/j.jmr.2012.09.007
A long-range optimized P.E.HSQC experiment, named P.E.HSQMBC, is proposed for the simultaneous measurement of a complete set of homonuclear and heteronuclear coupling constants from a single 2D cross-peak. The sign and the magnitude of proton–proton coupling constants are measured along the direct dimension from the relative E.COSY-type multiplet pattern displacement due to the passive one-bond coupling constant splitting generated in the indirect dimension. On the other hand, long-range proton–carbon coupling constants are independently determined in the detected dimension from a traditional fitting analysis of antiphase multiplet patterns or, more conveniently, from the IPAP multiplet displacement obtained from extended HSQMBC experiments.
Pulse Programs Code for Bruker:
- Non-Refocused Fully-Coupled P.E.HSQMBC Sequence
- Non-Refocused Fully-Coupled P.E.HSQMBC Sequence with 1J(CH)-scalable BIRD
- Refocused IPAP Version with 1J(CH)-scalable BIRD
