Perfect-HSQC experiments: pure in-phase spectra

cover“Suppresión of phase and amplitude JHH modulations in HSQC experiments” by Laura Castañar, Eduard Sistaré, Albert Virgili, Robert Thomas Williamson and Teodor Parella. Magnetic Resonance in Chemistry, 2014 , 53:115-119. DOI: 10.1002/mrc.4149

The amplitude and the phase of cross peaks in conventional 2D HSQC experiments are modulated by both proton–proton, JHH, and proton–carbon, 1JCH, coupling constants. It is shown by spectral simulation and experimentally that JHH interferences are suppressed in a novel perfect-HSQC pulse scheme that incorporates perfect-echo INEPT periods. The improved 2D spectra afford pure in-phase cross peaks with respect to 1JCH and JHH, irrespective of the experiment delay optimization. In addition, peak volumes are not attenuated by the influence of JHH, rendering practical issues such as phase correction, multiplet analysis, and signal integration more appropriate.


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