Discrimination of stereoisomers with RDCs

“Structural discrimination from in-situ measurement of 1DCH and 2DHH RDCs” by Núria Marcó,  R. R. Gil and Teodor Parella. Magnetic Resonance in Chemistry 2017, DOI: 10.1002/mrc.4575

A fast RDC-assisted strategy involving the simultaneous determination of scalar and total coupling constants from a single 1JCH/2JHH-resolved NMR spectrum is reported. It is shown that the concerted use of the directly measured 1DCH (for all CHn multiplicities) and 2DHH residual dipolar couplings allows an on-the-fly assignment of diastereotopic CH2 protons, as well as of an efficient discrimination between all eight possible diastereoisomeric structures of strychnine, which contains six stereocenters.

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