García Martín, María Luisa & Pilar López Larrubia (eds.). 2018. Preclinical MRI. Methods in Molecular Biology series. Springer New York. DOI: 10.1007/978-1-4939-7531-0
This book was conceived with the idea of providing an update on a wide variety of preclinical MRI methods and protocols to help technicians and researchers interested in this technology. The basics of MRI physics are introduced, followed by chapters describing updated methodology and protocols for some standard and more advanced MRI techniques covering diffusion, perfusion, functional imaging, in-vivo spectroscopy (proton and heteronuclear), susceptibility contrast MRI… The book also contains some chapters where some applications of those methods are illustrated in animal models of several diseases including cancer, stroke and neurodegeneration. Protocols are described in a step-by-step approach, with interesting notes and tips at the end of each chapter, which -a priori- should allow the new worker to obtain successful results with the first attempt ;o) .
On 21st March 2018 I sucessfully defended my PhD Thesis entitled: “New Applications of Covariance NMR and Experimental Development for Measurements of Homonuclear Coupling Constants in Overlapping Signals” (ISBN: 9788449079252), supervised by Dr. Teodor Parella and Dr. Pau Nolis, and obtained the degree of Ph.D. in Chemistry at the Department of Chemistry, Universitat Autònoma de Barcelona.
The experimental results obtained in this thesis are presented in the form of three papers published in NMR specialised scientific peer-reviewed journals.
Exploring the use of Generalized Indirect Covariance to Reconstruct Pure shift NMR Spectra: Current Pros and Cons. André Fredi, Pau Nolis, Carlos Cobas, Gary E. Martin and Teodor Parella. Journal of Magnetic Resonance, Volume 266, May 2016, Pages 16-22. DOI: 10.1016/j.jmr.2016.03.003
Access to experimentally infeasible spectra by pure-shift NMR covariance. André Fredi, Pau Nolis, Carlos Cobas and Teodor Parella. Journal of Magnetic Resonance, Volume 270, September 2016, Pages 161-168. DOI: 10.1016/j.jmr.2016.07.010
Accurate measurement of JHH in overlapped signals by a TOCSY‐edited SERF Experiment. André Fredi, Pau Nolis and Teodor Parella. Magnetic Resonance in Chemistry, Volume 55, Issue 6, June 2017, Pages 525-529. DOI: 10.1002/mrc.4572
The first two articles deal with the use of covariance NMR as a general method to generate novel psNMR spectra. The last work describes a new selTOCSY G-SERF experiment, for accurately measuring JHH in overlapped regions.
The first publication describes a novel general protocol to generate psNMR spectra by Covariance NMR. This new approach is unique in NMR spectroscopy; giving a cheap, fast an easy way to reconstruct psNMR spectra without spending time in the spectrometer. This new strategy has been referenced to as psNMR Covariance.
The concept of psNMR Covariance has been extended in the second publication by inserting Multiplicity-Edited (ME) information into 2D experiments that are difficult or even impossible to achieve experimentally. It is shown how the ME information can be efficiently transferred to a set of homonuclear and heteronuclear 2D NMR spectra by Covariance processing, reconstructing new psME spectra in a fast way. Finally, G-SERF and related methods only work for isolated 1H signals on which selective excitation can be successfully applied.
Unfortunately, as it happens in other frequency-selective experiments, this approach fails for overlapped signals. A doubly-selective TOCSY G-SERF scheme is presented in the third publication to circumvent this limitation, by measuring JHH efficiently even for protons resonating in crowded regions.
Last December, Esperança Ramírez, our facility secretary, defended her PhD Thesis entitled: “Edició crítica dels escolis al cant IX de l’Odissea” (Critical Edition of the Scholia to the Odyssey Book IX). The thesis defense took place at 11am on Friday 16 December in the Facultat de Filosofia i Lletres of Universitat Autònoma de Barcelona.
Scholia (singular scholium or scholion, from Ancient Greek: σχόλιον, “comment, interpretation”) are grammatical, critical, or explanatory comments, either original or extracted from pre-existing commentaries, which are inserted on the margin of the manuscript of an ancient author, as glosses. Scholia definition in the Wikipedia
In her doctoral dissertation she does a thorough, critical and updated edition of the scholia to the Odyssey Book IX, including the scholia regarded as minor as well as the major scholia. This book and the following three books are collectively referred to as the apologoi: Odysseus’ “stories”, and they have attracted the attention of scholars of Homer works of all times.
To carry out her research work, she had to systematically analyse and challenge previous editions that, in full or in part, included scholia from the Odyssey Book IX, and, on the other hand, she had to decide the corpus of manuscripts that she would study, which was finally set to 37 from different typologies and genetic filiations.
It stands out the methodological novelty of classifying the scholia according to their content: related to the titles, dedicated to argument summaries, focused on lexicographic aspects, contextual or mythographic. This classification, on top of helping to understand the content of each scholium, offers too an interesting view of the filiation of manuscripts.
Her goal was to offer a body of data encompassing the scholia, the annotations and also the most significant corrections of the Homeric text. In that corpus there are several types of content, from multiple versions of myths to words that must be construed, including syntactic expressions or morphological annotations.
Workshop limited to 4 participants (first come, first served)
Silvia Lope-Piedrafita, PhD ()
This course combines a comprehensive series of lectures on the technology of Magnetic resonance spectroscopy and imaging (MRS/MRI) with hands-on laboratory sessions to provide practical demonstrations of key concepts and procedures for preclinical studies.
Whether you are considering MRI as a research tool in your lab or just would like to learn more about MRI, this workshop addresses practical aspects of experimental MRI with laboratory animals and provide valuable hands-on experience on a 7 Tesla Bruker BioSpec spectrometer.
Abstract: Oocytes recovered from prepubertal goats are very heterogeneous in growth and grade of atresia which make them unpredictable for IVEP programs. We have observed that oocytes from prepubertal goats obtained from >3 mm follicles develop up to blastocyst stage at a similar percentage than oocytes from adult goats (18% vs 21%), suggesting that the follicle development and the follicular fluid (FF) content are more relevant to oocyte competence than the age of the donor. The aim of this study is to characterize the FF metabolomic profile from different follicular environments through a high-resolution 1H NMR-based metabolomic study. Samples of adult (n=40) and prepubertal (n=16) FF where collected by laparoscopic ovum pick-up (LOPU) and by aspiration of slaughterhouse ovaries, respectively. FF from small (< 3 mm) and large (> 3 mm) diameter follicles where pooled for each female. Multivariate ordination principal component analysis (PCA) was performed to detect patterns of sample ordination in the metabolomes. The unsupervised method clearly differed between the FF metabolomes of large and small follicles of prepubers and between the FF of preadolescent and adult individuals.
Figure. a) PCA scores plot (PC1-PC2) from 1H NMR spectral data of follicular fluid samples of preadolescent (n=16; blue dots) and adult (n=40; black dots) goats. b) PCA heat map loadings plot (PC1-PC2) with some discriminant variables assigned.
The researcher will investigate the subject “Analysis of complex mixtures by NMR spectroscopy” under the joint supervision of Dr. Teodor Parella and Dr. Míriam Pérez-Trujillo, as part of the project “Design and Application of New Methodologies in Nuclear Magnetic Resonance” (Diseño y aplicación de nuevas metodologías en resonancia magnética nuclear, CTQ2015-64436-P, 2016-2018) awarded to Dr. Teodor Parella.
An extraordinary atlas of mouse anatomy which includes more than 2,200 original images over 600 pages to show the anatomy, histology and cellular structure of mouse organs. This book attempts to provide an overview of the different levels of morphology of the mouse, ranging from gross anatomy and topographical anatomy (to explain the relative position of the organs and structures of a particular body region) down to the microscopic anatomy. Imaging technologies used for that include magnetic resonance imaging (MRI), computed tomography (CT), ultrasonography, angiography, X-ray, and electron microscopy. Also, classical anatomical techniques such as conventional dissection, skeletal preparations, vascular injections, histology and immunohistochemistry have been employed to characterize the mouse morphology.
All MRI images included in this book were acquired at our NMR facility (SeRMN, Universitat Autònoma de Barcelona) in a 7 Tesla Bruker BioSpec spectrometer.
André has been working as a Ph.D. candidate at the Department of Chemistry and SeRMN under the direction of Dr. Teodor Parella and Dr. Pau Nolis since November 2014, when he enrolled in the Department of Chemistry doctoral program at Universitat Autònoma de Barcelona with a fellowship from CNPq-Brazil. He is currently in his second year and expects to defend the doctoral thesis on 2017/2018.